ISSN 1119-4618
 

Original Research 


AMPEROMETRIC IMMUNOSENSOR BASED ON CONDUCTING LAYER OF PAni/Fe3O4 NANOCOMPOSITES FOR DETECTION OF Aβ42

Auwal Adamu Mahmoud, Mohd Kamarulzaki Mustafa, Nurun Najwa Ruslan, Aliyu Adamu Jauro, Ibrahim Hassan Garba, Umar Farouk Hassan.

Abstract
Conducting PAni/Fe3O4 nanocomposites were synthesized and electrochemically deposited on FTO coated glass to form a transducing layer owing to their catalytic, magnetic and electrical properties. This synthesized conducting layer was used to immobilized anti-β42 for detection of Alzheimer’s disease. The electrode modification process was characterized by electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV) and Field emission scanning electron microscopy (FESEM). some factors influencing the performance of the amperometric immunosensor were optimized. The experiment outcome indicates a conducting layer of the nanocomposites on the FTO coated glass at range of -1.5 to 0.5V at 0.10Vs-1 in a solution 10 ml of PAni-Fe3O4 nanocomposites. Electrochemical impedance changes occur only after PAni/Fe3O4 bound to surface of bare FTO, confirming the deposition and indicating that the new modified electrode can be used as a transducing matrix for the immobilization of biomarker. Tests performed with this immunosensor showed good linearity with a detection limit of 0.061 µg/mL-1 at 3σ

Key words: Immunosensor , Polyaniline , Nanocomposites , Conductivity , Fe3O4 , β-Amyloid


 
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How to Cite this Article
Pubmed Style

Mahmoud AA, Mustafa MK, Ruslan NN, Jauro AA, Garba IH, Hassan UF, . AMPEROMETRIC IMMUNOSENSOR BASED ON CONDUCTING LAYER OF PAni/Fe3O4 NANOCOMPOSITES FOR DETECTION OF Aβ42. JPAS. 2022; 22(2): 326-335. doi:10.5455/sf.99872


Web Style

Mahmoud AA, Mustafa MK, Ruslan NN, Jauro AA, Garba IH, Hassan UF, . AMPEROMETRIC IMMUNOSENSOR BASED ON CONDUCTING LAYER OF PAni/Fe3O4 NANOCOMPOSITES FOR DETECTION OF Aβ42. https://www.atbuscienceforum.com/?mno=99872 [Access: May 14, 2022]. doi:10.5455/sf.99872


AMA (American Medical Association) Style

Mahmoud AA, Mustafa MK, Ruslan NN, Jauro AA, Garba IH, Hassan UF, . AMPEROMETRIC IMMUNOSENSOR BASED ON CONDUCTING LAYER OF PAni/Fe3O4 NANOCOMPOSITES FOR DETECTION OF Aβ42. JPAS. 2022; 22(2): 326-335. doi:10.5455/sf.99872



Vancouver/ICMJE Style

Mahmoud AA, Mustafa MK, Ruslan NN, Jauro AA, Garba IH, Hassan UF, . AMPEROMETRIC IMMUNOSENSOR BASED ON CONDUCTING LAYER OF PAni/Fe3O4 NANOCOMPOSITES FOR DETECTION OF Aβ42. JPAS. (2022), [cited May 14, 2022]; 22(2): 326-335. doi:10.5455/sf.99872



Harvard Style

Mahmoud, A. A., Mustafa, M. K., Ruslan, N. N., Jauro, A. A., Garba, I. H., Hassan, U. F. & (2022) AMPEROMETRIC IMMUNOSENSOR BASED ON CONDUCTING LAYER OF PAni/Fe3O4 NANOCOMPOSITES FOR DETECTION OF Aβ42. JPAS, 22 (2), 326-335. doi:10.5455/sf.99872



Turabian Style

Mahmoud, Auwal Adamu, Mohd Kamarulzaki Mustafa, Nurun Najwa Ruslan, Aliyu Adamu Jauro, Ibrahim Hassan Garba, Umar Farouk Hassan, and . 2022. AMPEROMETRIC IMMUNOSENSOR BASED ON CONDUCTING LAYER OF PAni/Fe3O4 NANOCOMPOSITES FOR DETECTION OF Aβ42. Science Forum (Journal of Pure and Applied Sciences), 22 (2), 326-335. doi:10.5455/sf.99872



Chicago Style

Mahmoud, Auwal Adamu, Mohd Kamarulzaki Mustafa, Nurun Najwa Ruslan, Aliyu Adamu Jauro, Ibrahim Hassan Garba, Umar Farouk Hassan, and . "AMPEROMETRIC IMMUNOSENSOR BASED ON CONDUCTING LAYER OF PAni/Fe3O4 NANOCOMPOSITES FOR DETECTION OF Aβ42." Science Forum (Journal of Pure and Applied Sciences) 22 (2022), 326-335. doi:10.5455/sf.99872



MLA (The Modern Language Association) Style

Mahmoud, Auwal Adamu, Mohd Kamarulzaki Mustafa, Nurun Najwa Ruslan, Aliyu Adamu Jauro, Ibrahim Hassan Garba, Umar Farouk Hassan, and . "AMPEROMETRIC IMMUNOSENSOR BASED ON CONDUCTING LAYER OF PAni/Fe3O4 NANOCOMPOSITES FOR DETECTION OF Aβ42." Science Forum (Journal of Pure and Applied Sciences) 22.2 (2022), 326-335. Print. doi:10.5455/sf.99872



APA (American Psychological Association) Style

Mahmoud, A. A., Mustafa, M. K., Ruslan, N. N., Jauro, A. A., Garba, I. H., Hassan, U. F. & (2022) AMPEROMETRIC IMMUNOSENSOR BASED ON CONDUCTING LAYER OF PAni/Fe3O4 NANOCOMPOSITES FOR DETECTION OF Aβ42. Science Forum (Journal of Pure and Applied Sciences), 22 (2), 326-335. doi:10.5455/sf.99872